|
Please have some idea of how much sample is in your tube and use the following
as a rough guide for pure compounds (of molecular weight 600 g/mol or less) on
the AVANCE 400.
| |
50 mg or more |
20 mg -50 mg |
10 mg -20 mg |
5 mg - 10 mg |
Less than 5 mg |
| Proton |
16 scans |
16 scans |
16 scans |
16 -32 scans |
32 - 128 scans |
| Carbon |
5 minutes |
20 minutes |
1 - 2 hours |
2 hours |
>1 hours (use other spectromeyter) |
| COSY |
5 minutes |
5 minutes |
10 minutes |
20 minutes or more |
1 hour or more |
| HMQC / HSQC |
10 minutes |
10 minutes |
20 minutes |
20 minutes or more |
1 hour or more |
| HMBC |
20 minutes |
20 minutes |
1 hour |
1 hour |
>1 hours (use other spectromeyter) |
| NOESY |
1 hour |
1 hour |
1 hour |
1 hour |
>1 hours (use other spectromeyter) |
| Phosphorus |
1 minute |
1 minute |
1 - 5 minutes |
1 - 10 minutes |
1 - 20 minutes |
| Fluorine |
1 minute |
1 minute |
1 minute |
1 - 5 minutes |
1 - 10 minute |
|
